BDNF-TrkB signalling depletion from the indirect pathway spiny projection neurons (iSPN) leads to age-dependent spontaneous hyperlocomotion.

Therefore, we have established a unique mouse model that provides a rare example of an age-dependent locomotor defect to identify the genes and associated molecular pathways relevant to maintaining locomotor control. Thus, we performed gene expression profiling analysis using data from RNA-seq of 2 different striatal populations at two time points in the presence and absence of the TrkB signalling. Comparative gene expression profiling analysis of RNA-seq data from a limited number of purified adult or aged murine brain neurons lacking BDNF-TrkB signalling versus control mice.

据此，我们建立了一种独特的小鼠模型，该模型为研究年龄依赖性运动缺陷提供了罕见的范例，可用于鉴定与维持运动控制相关的基因及关联分子通路。因此，我们利用两种不同纹状体细胞群在两个时间点、存在或缺失酪氨酸激酶受体B（TrkB）信号时的RNA测序（RNA-seq）数据，开展基因表达谱分析。本研究针对少量纯化的成年或老年小鼠脑神经元的RNA测序数据开展比较基因表达谱分析，实验对象分为缺失脑源性神经营养因子（Brain-Derived Neurotrophic Factor，BDNF）介导的TrkB信号通路的小鼠，与对照小鼠。