Ip, Wallace (2010) CIL:2876, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in 0.2% saponin. The fibers were quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:2875.

兔子的腰大肌肌纤维在0.2%皂素溶液中保持恒定长度并诱导僵硬。肌纤维通过与液氦冷却的铜块接触进行快速冷冻，并在Heuser型冷冻压机中进行冷冻蚀刻。随后，样本在24摄氏度下以Pt-C进行旋转复制，并在操作于100 kev的JEOL 100CX2透射电子显微镜下进行可视化。图像以100,000倍放大率记录在Kodak 4489胶片上。在+5和-5度的角度下拍摄了立体对图像。使用Nikon Coolscan 9000ED扫描仪以20微米像素间距对胶片进行扫描。与CIL:2875的立体对图像一同进行扫描。