Laser dissection assisted transcriptomics reveals the role of the phloem in coordination of oilseed rape (Brassica napus L.) responses to clubroot disease

Phloem regions from Plasmodiophora brassicae infected and control, mock-inoculated plants were collected from the hypocotyls, and from the petioles of the first fully expanded true leaves, of Brassica napus cultivar Harry plants at 7 days and 12 days post-inoculation. RNA-Seq libraries were prepared from RNA which was extracted from laser-capture microdissection of phloem tissue from transverse sections.

本数据集的样本采集自甘蓝型油菜（Brassica napus）品种Harry的植株：分别在接种芸苔根肿菌（Plasmodiophora brassicae）以及进行空白模拟接种（对照组）后7天、12天，采集其下胚轴与第一片完全展开真叶叶柄处的韧皮部区域。随后通过激光捕获显微切割（Laser-capture microdissection）分离横切片中的韧皮部组织，提取总RNA并构建RNA测序（RNA-Seq）文库。