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Gene expression analysis of SHH-activated immortalized cranial neural crest cells (O9-1)

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE109432
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We sought to identify Hedgehog-regulated genes in mouse cranial neural crest cells (O9-1) Mouse cranial neural crest cells (O9-1) were allowed to attach for 24 hrs and media were replaced with DMEM containing 1% FBS ± SHH-N peptide at 0.4 μg/mL. 48 hrs after treatment, cells were lysed and RNA was collected per the GE Illustra RNAspin kits (GE Healthcare) general protocol for a cell monolayer.
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2018-05-02
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