Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model [RNA-seq]

Human embryonic stem cells were converted into GATA3-expressing extraembryonic cells (GATA3+ ExE cells) when they were exposed to chemical inhibitors for ACTIVIN/NODAL (A83-01, 1uM) and FGF signaling (PD03714, 100nM). We refer here to this inhibitor-induced differentiation method as an 'AP' protocol. To understand the transcriptome dynamics during AP-induced differentiation, we performed timecourse profiling of genome-wide gene expression by RNA sequencing. To understand transcriptome dynamicts during trophoblast-like differentiation, GATA3 reporter human ESCs were exposed to AP for 0-8 days.

将人类胚胎干细胞（human embryonic stem cells）暴露于针对激活素/结节蛋白（ACTIVIN/NODAL）的化学抑制剂A83-01（1μM）及FGF信号通路抑制剂PD03714（100nM）后，可将其转化为表达GATA3的胚外细胞（GATA3+ ExE细胞）。本研究将该抑制剂诱导的分化方法命名为"AP"方案。为解析AP诱导分化过程中的转录组动态变化，我们通过RNA测序对全基因组基因表达开展了时序谱分析。为探究滋养层样分化过程中的转录组动态变化，我们将携带GATA3报告基因的人类胚胎干细胞（human ESCs）置于AP方案中处理0至8天。