Long RNA-Sequencing Analysis of Extracellular Vesicles secreted by colorectal cancer cells

Although much is known about extracellular miRNA, the identity, regulation, and functional roles of secreted coding and long noncoding RNAs (lncRNAs, >200nt) are largely unknown. We have previously shown that mutant KRAS colorectal cancer (CRC) cells release EVs containing distinct proteomes, miRNAs, and circular RNAs. Here, we comprehensively identify the broad and diverse classes of CRC extracellular long RNAs secreted in EVs and demonstrate differential export of specific RNAs. We show that distinct noncoding RNAs including antisense transcripts and transcripts derived from pseudogenes are enriched in EVs compared to cellular profiles. Raw data from sequencing and analysis can be found at the supplied link (including the FASTQ data files).

尽管细胞外微小RNA（microRNA, miRNA）的相关研究已较为透彻，但分泌型编码RNA与长链非编码RNA（long noncoding RNAs, lncRNAs，长度>200nt）的鉴定、调控机制及功能作用仍尚不明确。我们此前的研究证实，携带KRAS突变的结直肠癌（colorectal cancer, CRC）细胞可释放含有独特蛋白质组、微小RNA及环状RNA的细胞外囊泡（extracellular vesicles, EVs）。本研究全面鉴定了结直肠癌来源、经细胞外囊泡分泌的各类广泛多样的长链细胞外RNA，并证实了特定RNA的差异化分选分泌过程。研究发现，相较于细胞内转录本，细胞外囊泡中富集了包括反义转录本及假基因来源转录本在内的独特非编码RNA类群。本研究的测序及分析原始数据（含FASTQ格式数据文件）可通过所提供的链接获取。