CIL:24046

Starved Dictyostelium cells lacking both guanylyl cyclases (GCase A and GCase), but expressing GCase with an inactivated catalytic domain (sGCΔCat) and in medium supplemented with 60 µM LY294002 (a PI3K inhibitor) and 1µM cAMP migrate continuously toward the anode (right). A direct-current electric field of 10 Vpcm was applied and the cells were observed for 20 min at 5-sec intervals. (Scale bar, 50 µm.) In contrast, cathode-directed migration is strongly attenuated in wild-type cells in the same medium. Compare with wild-type control video (CIL# 24043) and other mutants lacking guanylyl cyclase activity (CIL#24044, 24045). Videos are supporting information in Proc Natl Acad Sci (2009). 106: 6667-6672.饥饿处理的盘基网柄菌（Dictyostelium）细胞同时缺失两种鸟苷酸环化酶（guanylyl cyclases，GCases A与GCase），但表达带有失活催化结构域的鸟苷酸环化酶（sGCΔCat），且培养于添加了60 μM LY294002（一种磷脂酰肌醇3-激酶（PI3K）抑制剂）与1 μM 环腺苷酸（cAMP）的培养基中，此类细胞会持续向阳极（右侧）迁移。实验施加了10 Vpcm的直流电场，以5秒为间隔对细胞进行共计20分钟的观测，标尺长度为50 μm。与之相对，在相同培养基中的野生型细胞，其向阴极的迁移行为会被显著减弱。可将本实验结果与野生型对照视频（CIL# 24043）以及其他缺失鸟苷酸环化酶活性的突变体视频（CIL#24044、24045）进行对比。相关视频作为补充材料刊载于《美国国家科学院院刊》（Proc Natl Acad Sci）2009年，第106卷，第6667-6672页。