Transcriptional response of small intestinal epithelial cells (S-IECs) to bacterial monocolonization. Mus musculus

Here we analyzed the transcriptional profile of S-IECs sorted from GF and monocolonized mice (C. histolyticum and B. adolescentis), as well as SPF mice colonized with SFB or not. Overall design: S-IECs were double-sorted from mice colonized with the respective bacterial species directly into TRIzol.本研究分析了从小鼠中分选得到的小肠上皮细胞（small intestinal epithelial cells, S-IECs）的转录谱，所涉小鼠包括无菌（germ-free, GF）小鼠、分别定植溶组织梭菌（Clostridium histolyticum, C. histolyticum）或青春双歧杆菌（Bifidobacterium adolescentis, B. adolescentis）的单菌定植小鼠，以及定植或不定植分段丝状细菌（segmented filamentous bacteria, SFB）的无特定病原体（specific pathogen-free, SPF）小鼠。整体实验设计：将分别定植对应菌种的小鼠的S-IECs经双重分选后，直接收集至TRIzol试剂中。