Metagenome assembled genomes co_2-Bin_16_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

利用CONCOCT工具在anvi’o v5.1平台中完成共组装序列的分箱：将长度≥2500 bp的支架序列聚类为宏基因组组装基因组（metagenome assembled genomes，简称MAGs），并在anvi’o平台内对分箱结果进行人工优化。此外，采用dRep v2.3.2工具对所得MAGs进行去冗余处理，通过设置参数"-comp 50 -con 10"并保留其余默认参数，筛选获得代表性基因组。