Data_Sheet_1_The anti-inflammatory effects of photobiomodulation are mediated by cytokines: Evidence from a mouse model of inflammation.docx

There is an urgent need for therapeutic approaches that can prevent or limit neuroinflammatory processes and prevent neuronal degeneration. Photobiomodulation (PBM), the therapeutic use of specific wavelengths of light, is a safe approach shown to have anti-inflammatory effects. The current study was aimed at evaluating the effects of PBM on LPS-induced peripheral and central inflammation in mice to assess its potential as an anti-inflammatory treatment. Daily, 30-min treatment of mice with red/NIR light (RL) or RL with a 40 Hz gamma frequency flicker for 10 days prior to LPS challenge showed anti-inflammatory effects in the brain and systemically. PBM downregulated LPS induction of key proinflammatory cytokines associated with inflammasome activation, IL-1β and IL-18, and upregulated the anti-inflammatory cytokine, IL-10. RL provided robust anti-inflammatory effects, and the addition of gamma flicker potentiated these effects. Overall, these results demonstrate the potential of PBM as an anti-inflammatory treatment that acts through cytokine expression modulation.目前亟需能够阻断或抑制神经炎症进程、预防神经元变性的治疗手段。光生物调节疗法（Photobiomodulation, PBM）是指利用特定波长光线开展治疗的手段，是一种已被证实具备抗炎功效的安全疗法。本研究旨在评估光生物调节疗法对脂多糖（Lipopolysaccharide, LPS）诱导的小鼠外周与中枢神经系统炎症的作用，以考察其作为抗炎治疗手段的应用潜力。在脂多糖刺激前，每日对小鼠施以30分钟的红光/近红外光（Red Light, RL）照射，或搭配40Hz伽马频率闪烁的红光照射，持续10天，可在脑部与全身层面发挥抗炎作用。光生物调节疗法可下调脂多糖诱导的、与炎性小体激活相关的关键促炎细胞因子IL-1β与IL-18的表达，并上调抗炎细胞因子IL-10的水平。单独红光照射即可展现出显著的抗炎功效，而搭配伽马频率闪烁则可进一步强化该抗炎效果。综上，本研究结果证实了光生物调节疗法作为抗炎治疗手段的潜力，其作用机制为调控细胞因子的表达。