Data from: Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 × 103, 2.4 × 103 and 0.2 × 103 M−1, for UA1, UA6 and UA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the λmax value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng µl−1 for UA1, UA6 and UA7, respectively.

DNA传感始终是生物技术研究者与其他科研人员满怀探索热忱的研究沃土。近期，本领域中我们开发出一类含偶氮（azo）与胍（guanidine）官能团的新兴分子。本研究中，我们合成了三种全新化合物（UA1、UA6与UA7），旨在探索其在醇介质中用于DNA传感的潜在应用。所合成的化合物通过元素分析、傅里叶变换红外光谱（FTIR）、紫外-可见光谱、氢核磁共振（1H NMR）与碳核磁共振（13C NMR）波谱法完成了结构表征。我们通过紫外-可见光谱法考察了这三种化合物的DNA传感潜力。为进一步阐明其与DNA的相互作用机理，我们通过电化学（循环伏安法（cyclic voltammetry））与流体力学（粘度法）相关实验展开了深入研究。实验结果表明，三种化合物均具备中等强度的DNA结合能力，UA1、UA6与UA7的结合常数分别为7.2×10³、2.4×10³与0.2×10³ M⁻¹。当与DNA结合后，化合物会发生肉眼可见的颜色变化，即最大吸收波长λmax处发生蓝移。上述结果证实，所合成的化合物具备作为DNA传感器的应用潜力，UA1、UA6与UA7的检测限分别为1.8、5.8与4.0 ng·μL⁻¹。