Dataset for: Life tables of Bactericera cockerelli at constant 5, 10, 15, 25 and 30ºC and two fluctuating temperatures in Ecuador
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https://data.cipotato.org/citation?persistentId=doi:10.21223/XZU3CP
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资源简介:
"Life tables were established at constant temperatures of 5, 10, 15, 25, and 30ºC and two fluctuating temperatures, infesting potato cuttings with adults collected in capsules from the mass B. cockerelli cage. In each life table, 45 potato cuttings from INIAP - variety CIP Libertad were infested.. 4 adults were placed per cutting, which remained for 24 hours in the growth chamber. At the end of 24 h, the adults were removed with the help of an entomological aspirator and returned to the mother colony. Oviposition was evidenced in the cuttings, which were marked with the help of indelible red and blue markers, thus having 2 eggs per cutting (30 individuals per repetition). There are 3 repetitions, in each repetition there are 15 cuttings in vials, 45 in total for each temperature and with a total of 90 individuals, the evaluation was daily, counting the number of days it takes to change from one phenological state to another. until each individual dies. The following data was collected: Development time, Development rate, Mortality of each individual, and of the adult the reproductive capacity of the female will be obtained sexually, and adult longevity."
本实验构建了5、10、15、25、30℃恒温及两个变温条件下的生命表,采用从B. cockerelli大规模饲养笼中采集的成虫接种马铃薯插条。每个生命表使用45根来自INIAP的CIP Libertad品种的马铃薯插条,每根插条接入4头成虫,置于生长箱中静置24小时。24小时后,使用昆虫吸虫管吸出成虫并放回原饲养种群。插条上出现产卵痕迹后,用不褪色红蓝记号笔进行标记,每根插条保留2粒卵(每个重复对应30头个体)。本实验设置3次生物学重复,每个重复包含15支装于试管的插条,因此每个温度条件下总计45支插条,对应总个体数90头。每日开展观测记录,统计各虫态转换所需时长,直至所有个体自然死亡。本次实验采集的数据包括:各虫态发育历期、发育速率、单头个体死亡率;针对成虫,将统计雌性成虫的繁殖能力与成虫寿命。
提供机构:
International Potato Center
创建时间:
2023-01-09



