Prediction of Arabidopsis candidate proteins for S-nitrosylation using the GPS-SNO 1.0 software.

Arabidopsis amino acid sequences were extracted from TAIR 10 database (www.arabidopsis.org) and analysed by GPS-SNO 1.0 software using medium threshold condition. The 10% of predicted sites with the highest prediction confidence were determined by ranking the prediction results according to the raw score divided by the threshold (Cutoff) for a particular cluster.Prediction of Arabidopsis candidate proteins for S-nitrosylation using the GPS-SNO 1.0 software.本研究从TAIR 10数据库（www.arabidopsis.org）中提取拟南芥氨基酸序列，采用GPS-SNO 1.0软件（GPS-SNO 1.0）以中等阈值条件开展分析。通过依据特定聚类的原始得分除以阈值（Cutoff）对预测结果进行排序，筛选出预测置信度最高的10%的预测位点，并借助该软件完成拟南芥候选蛋白的S-亚硝基化（S-nitrosylation）预测。