Ionizing Radiation Induced Gene Expression Alterations in Human Peripheral Blood Mononuclear Cells. Homo sapiens

Ionizing Radition is known to cause cell damage. Human peripheral blood mononuclear cells have long been used to study radiation induced gene expression profiling. Within this study we evaluated gene and microRNA expression alterations of human PBMC irradiated with 60 Gy g-ray. Cells were cultured for 2, 4 and 20h after irradiation before RNA was isolated and Agilent whole human GenomeOligo Microarray was performed. Overall design: Radiation induced gene expression in human peripheral blood mononuclear cells were measured at 2, 4 and 20 hours after exposure to doses of 60 Gy g-rays. Non radiated cell of each donor and time point were used as contol cells. Four independent experiments were performed at each time (2, 4, or 20 hours and 0 h - only 1 sample per donor) using 4 different donors.

电离辐射（Ionizing Radiation）已知可引发细胞损伤。人类外周血单个核细胞（peripheral blood mononuclear cells, PBMC）长期被用于辐射诱导基因表达谱的相关研究。本研究针对经60戈瑞（Gy）γ射线照射的人类外周血单个核细胞，评估其基因与微小RNA（microRNA）的表达变化情况。细胞于辐照后分别培养2、4和20小时，随后提取RNA并开展安捷伦全人类基因组寡核苷酸微阵列（Agilent whole human Genome Oligo Microarray）检测。

实验设计：在暴露于60戈瑞γ射线后的2、4和20小时，检测人类外周血单个核细胞的辐射诱导基因表达水平。以未接受辐照的同供体、同时间点细胞作为对照。每个时间点（2、4、20小时以及0小时，其中0小时组每个供体仅设置1个样本）均开展4次独立实验，共使用4名不同供体的样本。