High sexual display trait diversity without measured genetic divergence in a montane hybrid zone involving young species (Habronattus americanus subgroup, Araneae, Salticidae)

Genetic introgression, allele exchange across species boundaries, is a commonly recognized feature of animal evolution. Under such a paradigm contemporary contact zones provide first-hand and complementary insight into the geographic, phenotypic, and genetic details of introgression. Also, when mate choice phenotypes are conspicuous and variable in hybrids, contact zones provide potential insight into how sexual selection interacts with species boundary maintenance, particularly when postzygotic reproductive isolation is weak. The Habronattus americanus subgroup includes several recently evolved jumping spider species, with an estimated age of about 200,000 years, and substantial evidence for hybridization and introgression. We explored a contact zone involving H. americanus (Keyserling, 1885) and H. kubai (Griswold, 1979) on Mount Shasta, California, in alpine habitats that would have been unavailable (under ice) at the Last Glacial Maximum. We characterized morphological diversity wit..., Specimen Collection. The Mount Shasta study site is located just west of Panther Meadows in the Shasta-Trinity National Forest, at an elevation of approximately 2300 meters, on the south flank of Mount Shasta. We surveyed for spiders in an approximately 270,000 square meter area. Habitat included a southward extending open area of mostly treeless high-elevation pumice field, with herbs and low shrubs, and adjacent high-elevation coniferous forest. A small, paved road winds through the study site. Habronattus americanus subgroup members prefer open habitats, so we spent most survey time out of the coniferous forest. We did not sample the more easterly Panther Meadows proper, with meadow edge microhabitats that we typically associate with H. kubai. Special permits were not required to collect spiders from this location.
Evidence of hybridization at this location was previously identified by the collection of male specimens with combined traits of both parental species. In July 2018 a team..., , # High sexual display trait diversity without measured genetic divergence in a montane hybrid zone involving young species (Habronattus americanus subgroup, Araneae, Salticidae)

## Description of the data and file structure

Supp. Table S1. Collection information and sequencing statistics for all specimens. Sample ID = voucher number for individual specimens; Morphological Analysis (x=included in analysis); ddRADSeq (x=included in analysis); palp color = intermediate highlighted in yellow; Raw reads & Loci - \"n/a\" specifies that RADSeq failed for specific specimen.

Supp. Table S2. Scored morphology matrix. Morphological scorings for individual specimens (see Supp Table S1), as follows:

**Character A** (Iridescent scales on clypeus).

* **States** 0 = no iridescent scales; 1 = full rectangular; 2 = ‘m’ shaped; 3 = ‘m’ shaped in middle, extends low to sides; 4 = four low semi-circle broken segments; 5 = two irregular iridescent patches separated at the center; 6 = very low ir...

遗传渐渗（genetic introgression）——即跨物种边界的等位基因交换——是动物演化领域公认的普遍特征。在此研究范式下，现存的杂交接触带可为渐渗过程的地理、表型及遗传细节提供一手且互补的研究视角。此外，若杂交个体的配偶选择表型显著且多样，杂交接触带则可用于探究性选择如何参与物种边界的维持，尤其当合子后生殖隔离较弱时。
美洲哈氏跳蛛类群（Habronattus americanus subgroup）包含数个新近演化的跳蛛物种，其分化时间估算约为20万年，且已有大量证据表明该类群存在杂交与遗传渐渗现象。我们以美国加利福尼亚州沙斯塔山的一处杂交接触带为研究对象，该生境为高山环境，在末次盛冰期曾被冰川覆盖，无法为蜘蛛提供生存空间，接触带涉及美洲哈氏跳蛛（H. americanus, Keyserling, 1885）与库巴哈氏跳蛛（H. kubai, Griswold, 1979）。我们对形态多样性进行了表征…… 标本采集。
本研究的沙斯塔山样地位于沙斯塔-特里尼蒂国家森林的潘瑟草甸西侧，海拔约2300米，地处沙斯塔山南坡。我们在面积约27万平方米的区域内开展蜘蛛调查。样地生境包括一片向南延伸的无树高海拔浮石荒滩，伴生草本与低矮灌木，以及毗邻的高海拔针叶林。一条小型铺装公路蜿蜒穿过样地。美洲哈氏跳蛛类群偏好开阔生境，因此我们的调查主要集中在针叶林外区域。我们未对更东侧的潘瑟草甸核心区域进行采样，该区域的草甸边缘微生境通常是库巴哈氏跳蛛的栖息生境。在此区域采集蜘蛛无需特殊许可。
此前已有研究通过采集兼具双亲性状的雄性标本，证实该区域存在杂交现象。2018年7月，某研究团队……
# 年轻物种山地杂交带中的性展示性状多样性与未检测到的遗传分化（美洲哈氏跳蛛类群，蜘蛛目，跳蛛科）

## 数据与文件结构说明
补充表S1：所有标本的采集信息与测序统计数据。样本ID=单个标本的凭证编号；形态学分析（x=纳入本分析）；双酶切RAD测序（ddRADSeq，x=纳入本分析）；触须颜色：黄色高亮标记的中间型；原始读长与位点——"n/a"表示该标本的RAD测序失败。
补充表S2：形态学性状评分矩阵。单个标本的形态学性状评分（详见补充表S1），具体如下：
**性状A**（额区（clypeus）上的虹彩鳞片）
* **状态** 0 = 无虹彩鳞片；1 = 完整矩形；2 = “M”形；3 = 中部为“M”形，向两侧向下延伸；4 = 四个低矮半圆形断裂片段；5 = 中央分隔的两处不规则虹彩斑块；6 = 极微弱的虹彩……