Ternary complex crystal structure of K289M variant of DNA polymerase Beta with "hot-spot sequence" with beta-gamma CHF analogue of dGTP

Ternary complex crystal structure of K289M variant of DNA polymerase Beta with "hot-spot sequence" with beta-gamma CHF analogue of dGTP Descriptor: 2'-DEOXY-5'-O-[(R)-{[(R)-[(R)-FLUORO(PHOSPHONO)METHYL](HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]GUANOSINE, CHLORIDE ION, DNA (5'-D(*CP*CP*GP*AP*AP*CP*AP*AP*GP*CP*AP*TP*CP*AP*GP*C)-3'), ... Authors: Batra, V.K, Wilson, S.H. Deposit date: 2019-01-07 Release date: 2020-01-08 Last modified: 2024-03-13 Method: X-RAY DIFFRACTION (2.094 Å) Cite: Revealing an Internal Stabilization Deficiency in the DNA Polymerase beta K289M Cancer Variant through the Combined Use of Chemical Biology and X-ray Crystallography. Biochemistry, 59, 2020

DNA聚合酶β（DNA polymerase Beta）K289M突变体与脱氧鸟苷三磷酸（dGTP）的β-γ CHF类似物、“热点序列”DNA形成的三元复合物晶体结构。配体与样本描述：2'-脱氧-5'-O-[(R)-{[(R)-[(R)-氟(膦酰)甲基](羟基)膦酰]氧基}(羟基)膦酰]鸟苷、氯离子；DNA序列为5'-D(*CP*CP*GP*AP*AP*CP*AP*AP*GP*CP*AP*TP*CP*AP*GP*C)-3'，原文存在省略内容。作者：Batra, V.K.、Wilson, S.H.。存档日期：2019-01-07；发布日期：2020-01-08；最后修改日期：2024-03-13。实验方法：X射线衍射（分辨率2.094埃）。引用文献：《通过化学生物学与X射线晶体学联用揭示DNA聚合酶β K289M癌症突变体的内部稳定缺陷》，发表于《Biochemistry》，2020年，第59卷。