scRNA-seq data from liver mononuclear cells after AhR activation. Mus musculus

We studied the effects of aryl hydrocarbon receptor (AhR) activation by a single injection of 2,3,7,8-Tetrachlorodibenzodioxin (TCDD), 6-Formylindolo[3,2-b]carbazole (FICZ), or vehicle (corn oil) on mouse liver mononuclear cells challenged with Concanavalin A (ConA) at a dose of 12.5 mg/kg. Treatment with TCDD or FICZ was given 1 hour after ConA and cells were isolated 24 hours after ConA. Overall design: 2-3 samples were analyzed from the naive, ConA+Veh, ConA+TCDD, ConA+FICZ groups. Naive were not given any compound, ConA+Veh is the ConA-exposed, vehicle control, ConA+TCDD is the group exposed to ConA that received TCDD, and ConA+FICZ is the group exposed to ConA that received FICZ.本研究旨在探讨单次注射2,3,7,8-四氯二苯并二噁英（2,3,7,8-Tetrachlorodibenzodioxin, TCDD）、6-甲酰基吲哚并[3,2-b]咔唑（6-Formylindolo[3,2-b]carbazole, FICZ）或溶剂（玉米油）以激活芳基烃受体（aryl hydrocarbon receptor, AhR），对以12.5mg/kg剂量刀豆蛋白A（Concanavalin A, ConA）刺激的小鼠肝脏单个核细胞的影响。实验中，TCDD或FICZ的给药时机为刀豆蛋白A刺激后1小时，细胞于刀豆蛋白A刺激后24小时分离获取。实验设计概述如下：设置4组实验，分别为未接受任何受试物处理的空白对照组（Naive组）、ConA+溶剂对照组（ConA+Veh组，即仅接受刀豆蛋白A暴露的溶剂对照）、ConA+TCDD处理组（接受刀豆蛋白A暴露并给予TCDD处理）以及ConA+FICZ处理组（接受刀豆蛋白A暴露并给予FICZ处理），每组采集2-3份样本进行分析。