Single-cell profiling of developing mouse lungs. Single-cell profiling of developing mouse lungs

Single-cell profiling of wild type lungs was performed to delineate the expression profile of epithelial, endothelial, mesenchymal and immune cells. Overall design: FACS-purified whole murine lung epithelial, endothelial, mesenchymal, and immune cells were mixed together in similar proportions and processed through the Chromium Single Cell Gene Expression Solution Platform (10X Genomics).本研究对野生型肺部开展单细胞分析（single-cell profiling），以阐明上皮细胞、内皮细胞、间充质细胞及免疫细胞的表达谱特征。总体实验设计：经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）纯化的全小鼠肺部上皮细胞、内皮细胞、间充质细胞与免疫细胞，按相似比例混合后，通过Chromium单细胞基因表达解决方案平台（10X Genomics）完成样本处理。