RNA-sequencing of WT and P2Y4 KO pericardial adipose tissue (PAT) 24 hours after left anterior descending artery (LAD) ligation

We identified differentially regulated genes in the pericardial adipose tissue of wild type (WT) and P2Y4 KO mice, 24 hours after left anterior descending artery (LAD) ligation as a model of myocardial infarction (MI). Differentially regulated genes were involved in metabolism, brown fat cell differentiation, immune response and inflammation. P2Y4 KO mice displayed adipocyte beiging with increased PD-L1 expression and a higher number of regulatory leukocytes in their PAT 24 hours after LAD ligation. Up-regulated genes linked to adipocyte beiging included Ucp1, Cited1 and Prdm16. 2 samples were analyzed, a pool of the RNA of 3 WT PAT and a pool of the RNA of 3 P2Y4 KO PAT. PAT was freshly harvested 24 hours after LAD ligation.

本研究以左前降支动脉（left anterior descending artery, LAD）结扎构建心肌梗死（myocardial infarction, MI）模型，于造模后24小时采集野生型（wild type, WT）与P2Y4敲除（P2Y4 knockout, KO）小鼠的心包脂肪组织（pericardial adipose tissue, PAT），并鉴定该组织中的差异表达基因。所获差异表达基因主要参与代谢过程、棕色脂肪细胞分化、免疫应答与炎症反应。P2Y4敲除小鼠在左前降支动脉结扎后24小时，其心包脂肪组织中可观察到脂肪细胞米色化现象，同时伴随PD-L1（Programmed Death-Ligand 1）表达上调且调节性白细胞数量增多。与脂肪细胞米色化相关的上调基因包括Ucp1、Cited1与Prdm16。本研究共分析2份样本，分别为3只野生型小鼠心包脂肪组织的RNA混合池与3只P2Y4敲除小鼠心包脂肪组织的RNA混合池；所有心包脂肪组织均于左前降支动脉结扎后24小时新鲜采集。