Genome-wide maps of m6dA bases in drosophila ovary genomic DNA

We report the m6dA modification on the Drosophila genome. We collected ovary genomic DNA from 2-day wild-type and DMAD mutant files and performed DNA-immunoprecipitation(DNA-IP)experiments using anti-m6dA antibody. The generated DNA library was subjected to a high-throughput deep sequencing analysis. In this assay, the IgG-immunoprecipited DNA from the same amount of wild-type ovaries was used as the control, and the high-throughput sequencing resulted in a range of approximately 3 to 4.6 million reads. In sum, we identified 50 and 195 peaks from wild-type and DMAD mutant samples. Importantly, m6dA is mainly utilized to modify the transposon sequence on the chromosomes. Overall design: Examination of m6dA modifications in Genomic DNA of WT and DMAD mutant ovary.

本研究报道了果蝇基因组中的m6dA修饰。我们从2日龄野生型与DMAD突变体果蝇的卵巢中提取基因组DNA，并使用抗m6dA抗体开展DNA免疫沉淀（DNA-immunoprecipitation，DNA-IP）实验。所构建的DNA文库随后被用于高通量深度测序分析。本实验中，我们以等量野生型卵巢的IgG免疫沉淀DNA作为对照，高通量测序共获得约300万至460万条读段（reads）。综上，我们分别从野生型与DMAD突变体样本中鉴定得到50个和195个富集峰。值得注意的是，m6dA修饰主要靶向修饰染色体上的转座子序列。实验设计：检测野生型（Wild-Type，WT）与DMAD突变体卵巢基因组DNA中的m6dA修饰。