Skull vault formation in individual Chi-/+ fish

An individual fish was imaged successively from 14-36 dpf by confocal microscopy, during the period of induction and growth of the frontal and parietal bones. The fish was transgenic for col1a1a:gfp, marking chondrocytes, and for sp7:mcherry, marking osteoblasts. The fish was a heterozygous mutant for Chihuahua, and mutation in the col1a1a gene that models human Osteogenesis Imperfecta.

本数据集采用共聚焦显微镜（confocal microscopy），在受精后14至36天（days post fertilization，简称dpf）的时段内对一条个体实验鱼进行连续成像，该成像周期覆盖额骨与顶骨的诱导生成及生长发育全过程。该鱼为col1a1a:gfp与sp7:mcherry双转基因品系，其中col1a1a:gfp可特异性标记软骨细胞（chondrocytes），sp7:mcherry可特异性标记成骨细胞（osteoblasts）。此外，该鱼同时为Chihuahua基因的杂合突变体，且携带col1a1a基因突变——该突变可精准模拟人类成骨不全症（Osteogenesis Imperfecta）的病理特征。