CLL patients with TP53 abnormalities.

The assessment of the functional status of P53 in CLL cells was based on induction of P21 and P53 protein expression using etoposide and nutlin-3a [39]. The detection of TP53 mutations was performed as described in Methods. FISH: score of interphasic cells with an abnormality. Del, deletion.CLL patients with TP53 abnormalities.本研究通过依托泊苷（etoposide）与Nutlin-3a诱导P21及P53蛋白表达，以此评估慢性淋巴细胞白血病（Chronic Lymphocytic Leukemia, CLL）细胞中P53的功能状态[39]。 TP53基因突变的检测参照本文"方法"部分所述流程完成。 荧光原位杂交（Fluorescence In Situ Hybridization, FISH）：对存在异常的间期细胞进行评分。 Del，即缺失（deletion）。 伴TP53异常的慢性淋巴细胞白血病患者。