Whole-genome sequencing of multi-drug resistant Escherichia coli from waste and river water. Whole-genome sequencing of multi-drug resistant Escherichia coli from waste and river water

Natural and human-related aquatic environments are subjected to different ecological factors that influenced the emergence, evolution and dissemination of threatening antibiotic resistance mechanisms for Public Health. 16S rRNA methyltransferases are one of these mechanisms, enzymes that confer resistance to all clinically relevant aminoglycosides, including plazomicin, a last resort antibiotic. This study was focused on the analysis of the bacterial, plasmidic and genetic structure of Escherichia coli populations harboring these resistance determinants, among others, from both waste and river waters located in the area of Barcelona (Spain). The results allowed the understanding of the different resistance gene dynamics and adaptation to the environment according to the ecological niche, identifying the processes that led to high-risk associations and its dissemination. All samples are related to complete genomic data and metadata which allow for future studies of these multi-drug resistant E. coli worldwide.

与自然及人类活动相关的水生环境，会受到各类生态因子的作用，这些因子可影响威胁公共卫生的抗生素耐药机制的产生、演化与传播。16S rRNA甲基转移酶（16S rRNA methyltransferase）即属于此类耐药机制，这类酶可使细菌对所有临床相关氨基糖苷类抗生素产生耐药性，其中包括普拉佐米星（plazomicin）——一种临床最后一线使用的抗生素。本研究聚焦于对西班牙巴塞罗那地区废水及河水中的大肠杆菌（Escherichia coli）种群展开分析，解析其携带的各类耐药决定因子（含上述16S rRNA甲基转移酶编码基因）的细菌、质粒及遗传结构特征。本研究结果阐明了不同耐药基因的动态变化规律及其依据生态位实现的环境适应性，并明确了促成高风险耐药关联及其传播的分子过程。本数据集所有样本均配套完整基因组数据与元数据，可为全球范围内针对此类多重耐药大肠杆菌的后续研究提供支撑。