MiRNA expression pattern in different prognosis of colon cancer patients. Homo sapiens

We measured the expression of human microRNAs in tumor cells derived from 8 FFPE samples among non-invasive CRC patients with different prognosis. Patients lived for more than 5 years after surgery were classified as good prognosis, and patients died within 5 years from surgery were classified as poor prognosis. Tumor tissues were macrodissected under the control HE staining slides. And there were at least 75 percent cancer cells in the samples. Overall design: MicroRNA microarray was used to measure the expression of human miRNAs in tumor cells derived from 8 FFPE samples among early stage CRC patients with different prognosis.

本研究对8份福尔马林固定石蜡包埋（Formalin-Fixed Paraffin-Embedded，FFPE）样本来源的肿瘤细胞中人微小RNA（microRNA）的表达水平进行了检测，上述样本取自预后存在差异的非侵袭性结直肠癌（Colorectal Cancer，CRC）患者。将术后存活时间超过5年的患者划分为预后良好组，将术后5年内死亡的患者划分为预后不良组。肿瘤组织以HE染色切片为参照进行大体剖离，且所有样本中的癌细胞占比均不低于75%。整体实验设计：本研究采用微小RNA微阵列，对8份来自预后不同的早期结直肠癌患者的福尔马林固定石蜡包埋样本来源的肿瘤细胞中人微小RNA的表达水平进行了检测。