Identification of GLYMA.04G093300 and GLYMA.06G314400 binding sites in soybean cotyledon stage embryos.. Identification of GLYMA.04G093300 and GLYMA.06G314400 binding sites in soybean cotyledon stage embryos.

To dissect the gene regulatory networks operating during soybean embryo development, we identified the binding sites genome-wide for transcription factor GLYMA.04G093300 and GLYMA.06G314400 in cotyledon stage embryos using ChIP-seq. Overall design: Identification of GLYMA.04G093300 and and GLYMA.06G314400 binding sites in soybean embryos at the cotyledon stage. ChIP and total chromatin controls (input) were obtained for two biological replicates. A total of 7 samples were sequenced using the Illumina NovaSeq 6000 platform.

为解析大豆胚胎发育过程中运作的基因调控网络，我们采用染色质免疫沉淀测序（ChIP-seq）技术，在子叶期胚胎中全基因组范围内鉴定了转录因子GLYMA.04G093300与GLYMA.06G314400的结合位点。实验总体设计：在大豆子叶期胚胎中鉴定转录因子GLYMA.04G093300与GLYMA.06G314400的结合位点。本实验设置染色质免疫沉淀（ChIP）与全染色质对照（Input）样本，共完成2次生物学重复。总计7份样本通过Illumina NovaSeq 6000平台完成测序。