Draft genomes of vinasse bacteria
收藏资源简介:
Twenty-one draft bacterial genomes and their annotations described in the publication Cassman, NA. et. al. 2018, Biotech for Biofuels. The goal of the study was to characterize the microbial assemblage present in sugarcane vinasse, which is the major waste of bioethanol production from sugarcane and generally used as an organic and/or K fertilizer. Briefly, the draft genomes were binned (maxbin2) and manually refined (anvi'o v.2.3.2) from a cross-assembly (metaSPADES v3.8.2) of 18 metagenomes sequenced from six batches of sugarcane vinasse with Illumina Miseq technology. Annotations (prokka v1.12) were carried out against prokka databases (UniProtKB Bacterial and the HAMAP HMM database) along with the dbCAN HMM database (dbCAN-fam-HMMs.v6). The uploaded files are from 1) prokka output: bin nucleotide sequences (fa), protein sequences (faa), annotations (gff and tsv) and annotation info (txt) and 2) anvi'o output: percent recruitment of the bins, bin summary and samples summary.
本数据集包含21份细菌草图基因组及其注释信息,相关研究成果发表于Cassman NA等人2018年发表于《生物燃料生物技术(Biotechnology for Biofuels)》的论文。本研究的目标为解析甘蔗酒糟中的微生物群落:甘蔗酒糟是甘蔗基生物乙醇生产的主要副产物,通常被用作有机肥料和/或钾肥。 简要来说,研究人员采用Illumina MiSeq测序技术对6批甘蔗酒糟样品进行测序,获得18份宏基因组数据;随后通过metaSPAdes v3.8.2完成共组装,再利用maxbin2进行基因组分箱,并借助anvi'o v2.3.2软件完成手动优化。 基因组注释采用prokka v1.12软件完成,注释数据库涵盖UniProtKB细菌蛋白数据库、HAMAP隐马尔可夫模型(Hidden Markov Model,HMM)数据库以及dbCAN隐马尔可夫模型数据库(dbCAN-fam-HMMs.v6)。 本次上传的文件包含两类:1)prokka输出文件:分箱的核苷酸序列(fa格式)、蛋白质序列(faa格式)、注释文件(gff与tsv格式)以及注释信息文本(txt格式);2)anvi'o输出文件:分箱招募百分比、分箱汇总信息及样品汇总信息。



