Ageing, TOR and amino acid restriction: a cross-tissue transcriptional network connects GATA factors to Drosophila longevity. Ageing, TOR and amino acid restriction: a cross-tissue transcriptional network connects GATA factors to Drosophila longevity

RNA libraries from whole D. melanogaster and dissected tissues (brain, fat body, gut, ovary, thorax). Flies were fed 1SY base food (10% yeast, 5% sucrose, 1.5% agar), EAA-enriched 1SY (equivalent to EAA content of 20% yeast as Emran et al, 2014: PMC4069266), or EAA-enriched 1SY + 200µm rapamycin

本数据集的RNA文库取自完整黑腹果蝇（Drosophila melanogaster）以及解剖分离的组织（大脑、脂肪体、肠道、卵巢、胸部）。实验果蝇分别被饲喂三种培养基：1SY基础培养基（含10%酵母、5%蔗糖、1.5%琼脂）、必需氨基酸（Essential Amino Acids）富集型1SY培养基（其必需氨基酸含量等同于20%酵母培养基，参照Emran等2014年研究：PMC4069266），以及添加200μM雷帕霉素的必需氨基酸富集型1SY培养基。