Design and Evaluation of Illumina MiSeq compatible 18S rRNA Primers for Improved Characterization of Mixed Phototrophic Communities.

A systematic analysis of the most commonly amplified 18S rRNA hypervariable regions and the effect that PCR/sequencing bias has on community representation. The V4 and V8-V9 hypervariable regions were amplified for seven mock communities and for freshwater, marine, and wastewater samples to compare the sequenced community structure to theoretical communities and validate these results with environmental samples.

针对最常被扩增的18S核糖体RNA（18S rRNA）高变区，以及聚合酶链式反应（Polymerase Chain Reaction，PCR）/测序偏倚对群落表征的影响开展系统性分析。研究人员对7组模拟群落以及淡水、海洋和废水样本进行V4与V8-V9高变区的扩增，以将测序得到的群落结构与理论群落进行比对，并利用环境样本对上述结果进行验证。