DataSheet1_Restorative Effects of Inulin From Codonopsis pilosula on Intestinal Mucosal Immunity, Anti-Inflammatory Activity and Gut Microbiota of Immunosuppressed Mice.pdf

An inulin (CPPF), isolated from a traditional Chinese herbal medicine Codonopsis pilosula, was characterized and demonstrated with potential prebiotic activity in vitro before. Based on its non-digested feature, the intestinal mucosa and microbiota modulatory effects in vivo on immunosuppressed mice were investigated after oral administration of 200, 100 and 50 mg/kg of CPPF for 7 days. It was demonstrated that the secretions of sIgA and mucin 2 (Muc2) in ileum were improved by CPPF, and the anti-inflammatory activities in different intestine parts were revealed. The intestine before colon could be the target active position of CPPF. As a potential prebiotic substance, a gut microbiota restorative effect was also presented by mainly modulating the relative abundance of Eubacteriales, including Oscillibacter, unidentified Ruminococcus and Lachnospiraceae after high-throughput pyrosequencing of V4 region of 16S rRNA analysis. All these results indicated that this main bioactive ingredient inulin from C. pilosula was a medicinal prebiotic with enhancing mucosal immune, anti-inflammatory and microbiota modulatory activities.

本研究针对从传统中药材党参（Codonopsis pilosula）中分离得到的菊糖（CPPF）展开分析，此前已有研究对其进行了表征，并证实其具备潜在的体外益生元活性。基于该物质不易被消化的特性，本研究以免疫抑制小鼠为模型，通过灌胃给予200、100、50 mg/kg剂量的CPPF并连续干预7天，探究了其对小鼠肠道黏膜及肠道菌群的体内调节作用。实验结果证实，CPPF可提升小鼠回肠中分泌型免疫球蛋白A（sIgA）及黏蛋白2（Muc2）的分泌水平，并在肠道不同区段展现出抗炎活性。研究发现，结肠上游的肠道区段或为CPPF的主要作用靶部位。作为一种潜在益生元物质，CPPF还具备肠道菌群修复功效：通过对16S rRNA的V4区进行高通量焦磷酸测序分析可知，其主要通过调节真杆菌目（Eubacteriales）下属的颤杆菌属（Oscillibacter）、未鉴定瘤胃球菌（unidentified Ruminococcus）及毛螺菌科（Lachnospiraceae）的相对丰度，实现对肠道菌群的调控。综合上述实验结果可知，党参中的这一主要活性成分菊糖CPPF是一种兼具黏膜免疫增强、抗炎及肠道菌群调节活性的药用益生元。