Gene Profiling in Mouse Fetal Ductus Arteriosus. Mus musculus

The ductus arteriosus (DA) is a fetal vascular shunt that is located between the main pulmonary artery and the aorta. Oxygenated fetal blood from the placenta is shunted past the uninflated fetal lungs, crosses the DA, and is then available to the peripheral organs. In utero closure of the DA is deleterious, but postnatal closure of the DA is necessary for establishment of pulmonary circulation and the transition to newborn life. We used microarrays to compare ductus arteriosis (DA) and aortic gene expression at a single time point (day 19 of gestation, which is the day of expected delivery). Overall design: DA and corresponding aortic vascular segments from fetal mice on day 19 of gestation were collected from every pup in a litter (up to the first 10 pups). These samples were pooled and considered representative of the DA's or aortas from one pregnant female. Tissues were homogenized via 27g needle, per GentraSystems Versagene kit

动脉导管（ductus arteriosus，DA）是一种胎儿时期的血管分流通道，坐落于主肺动脉与主动脉之间。来自胎盘的含氧胎儿血液绕过未扩张的胎儿肺部，经动脉导管分流后，可供外周器官利用。子宫内动脉导管闭合会造成不良影响，但出生后动脉导管闭合对于建立肺循环、完成向新生生命的过渡是必需的。本研究采用基因芯片（microarrays），在单个时间点（妊娠第19天，即预期分娩日）比较动脉导管与主动脉的基因表达水平。实验整体设计：收集妊娠第19天胎鼠的动脉导管及对应主动脉血管节段，每窝幼鼠全部采样（最多取前10只）。将采集的样本混合，视为代表单只孕鼠的动脉导管或主动脉组织。按照GentraSystems Versagene试剂盒的操作流程，使用27G针头对组织进行匀浆处理。