Gene expression profile of cancer cells growing as a co-culture with carcinoma-associated fibroblasts (CAF). Canis lupus familiaris

Molecular mechanisms of the cancer cells-carcinoma associated fibroblasts (CAF) interactions growing in vitro conditions as a co-culture. The five canine mammary cancer cell lines were cultured with CAF (isolated from canine mammary cancer) for 72hrs. Then, the cancer cells and CAFs were sorted and the gene expression analysis was conducted. The control for each co-cultured cell line was the same cell line growing as a single culture. Overall design: Dye-swap experiment, each cell line growing in the co-culture conditions was compared to the same cell line growing as a single culture.

本数据集围绕体外共培养条件下癌细胞与癌相关成纤维细胞（carcinoma associated fibroblasts, CAF）的相互作用分子机制展开。实验选取5株犬乳腺癌细胞系，与从犬乳腺癌组织中分离得到的癌相关成纤维细胞共培养72小时；随后对癌细胞与癌相关成纤维细胞进行分选，并开展基因表达分析。每个共培养细胞系的对照组为单独培养的同株细胞系。实验总体设计采用染料互换（dye-swap）实验方案，将共培养条件下的各细胞系与单独培养的同株细胞系进行对比分析。