Microbial Community Dynamics Analysis Using 338F-806R Primer Sequences in Soil Samples

This study aims to investigate the temporal dynamics of microbial communities in soil samples through high-throughput sequencing of the 16S rRNA gene region amplified by 338F-806R primers. The research focuses on comparing microbial diversity across different treatment groups (e.g., CK, MR, C1, C3, C6) at multiple time points (1-day, 7-day, 30-day intervals). The generated FASTQ files (R1/R2 pairs) will enable taxonomic profiling and ecological insights into how environmental factors or interventions influence soil microbiota. Findings will contribute to understanding microbial succession patterns and functional adaptations in terrestrial ecosystems, with potential applications in agriculture, bioremediation, and ecosystem health monitoring.本研究旨在通过对338F-806R引物扩增的16S rRNA基因（16S rRNA gene）区域开展高通量测序（high-throughput sequencing），探究土壤样本中微生物群落的时间动态变化规律。研究聚焦于在多个时间节点（1天、7天、30天间隔）下，对比不同处理组（如CK、MR、C1、C3、C6）的微生物多样性差异。所生成的FASTQ文件（R1/R2双端序列对）可支撑分类学谱系分析，并为解析环境因子或干预手段对土壤微生物群落的影响提供生态学洞察。本研究结果将有助于深入理解陆地生态系统中的微生物演替模式与功能适应性，其成果有望在农业、生物修复及生态系统健康监测等领域得到应用。