Hedgehog signaling affects platelet-derived growth factor signaling mediated alveolar septum formation during postnatal lung development [scRNA-seq]

HH inhibition reproduces the lung phenotype of PDGF pathway knockout lungs and results in profound effects on differentiation and gene expression of Pdgfra-expressing alveolar mesenchymal cells, in particular myofibroblasts. Overall design: Whole lung single cell suspensions of Gli1creERT2/+;R26RtdTom/+ reporter lungs (P6) from mice, labelled with tamoxifen at P1 followed by treatment with anti-HH antibody 5E1 or control IgG at P3 were loaded onto a GEM code instrument (10xGenomics).

刺猬信号通路（HH，Hedgehog）抑制可重现血小板衍生生长因子（PDGF，Platelet-Derived Growth Factor）通路基因敲除小鼠的肺表型，并对表达Pdgfra（Platelet-Derived Growth Factor Receptor Alpha）的肺泡间充质细胞（尤其是肌成纤维细胞）的分化与基因表达产生显著影响。整体实验设计：选取出生后第6天（P6）的Gli1creERT2/+;R26RtdTom/+报告基因小鼠，于出生后第1天（P1）经他莫昔芬进行细胞标记，随后在出生后第3天（P3）分别采用抗HH抗体5E1或对照免疫球蛋白G（IgG）处理，制备其全肺单细胞悬液并加载至GEM编码仪（10xGenomics公司）开展后续检测。