Differential expression of extracellular vesicles-derived long RNA sequencing of plasma from patients after myocardial infarction with or without cardiac remodeling and healthy individuals

The goals of this study are to compare extracellular vesicles-derived circRNAs-lncRNAs-mRNA(RNA-seq) from the plasma of myocardial infarction patients with cardiac remodeling (C), without cardiac remodeling (NC), and normal healthy people (N). Human plasma was separated by centrifugation at 3,000 rpm for 10 min at room temperature (25°C) within 2 h after blood collection and then centrifuged at 13,000 rpm for 10 min at 4°C to remove debris. Isolated plasma samples were stored at -80°C until use. Extracellular vesicles RNA were isolated by affinity-based binding to spin columns using an exoRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany). RNA libraries were prepared for sequencing using SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. RNA-seq was performed by the Illumina NovaSeq 6000 on a 150-bp paired-end run. Extracellular vesicles-derived long RNA profiles of patients after myocardial infarction with cardiac remodeling (C), without cardiac remodeling (NC) and normal healthy individuals (N) by deep sequencing using Illumina NovaSeq 6000

本研究旨在对比心肌梗死伴心脏重构患者（C组）、不伴心脏重构患者（NC组）及正常健康人群（N组）血浆来源的细胞外囊泡（extracellular vesicles）环状RNA（circRNAs）-长链非编码RNA（lncRNAs）-信使RNA（mRNA）的RNA测序（RNA-seq）结果。血液采集后2小时内，于室温（25℃）以3000转/分钟离心10分钟分离得到人血浆，随后在4℃条件下以13000转/分钟离心10分钟去除细胞碎片。分离得到的血浆样本保存于-80℃冰箱直至实验使用。采用德国希尔德市凯杰（Qiagen, Hilden, Germany）公司的exoRNeasy血清/血浆试剂盒，通过亲和结合离心柱法分离细胞外囊泡RNA。使用SMARTer链特异性总RNA测序试剂盒——微量哺乳动物样本版（SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian）构建RNA测序文库。采用Illumina NovaSeq 6000测序平台，以150bp双端测序模式完成RNA测序。本研究通过该平台开展深度测序，成功获取了心肌梗死伴心脏重构患者（C组）、不伴心脏重构患者（NC组）及正常健康个体（N组）的细胞外囊泡来源长RNA表达谱。