Swine fecal microbiota with or without G-CSF and Salmonella challenge Targeted loci

Swine were administered G-CSF prior to Salmonella enterica serovar Typhimurium challenge to test whether an increase in circulating neutrophil populations could mitigate Salmonella shedding in feces. Fecal samples were taken immediately prior to and up to seven days following Salmonella challenge. The microbiota was assessed via the V1-V3 region of the 16s rRNA gene.本研究在鼠伤寒沙门氏菌（Salmonella enterica serovar Typhimurium）攻毒前，对实验猪施用粒细胞集落刺激因子（Granulocyte Colony-Stimulating Factor, G-CSF），旨在验证循环中性粒细胞群体的增加是否可减轻粪便中的沙门氏菌排菌情况。研究人员分别于沙门氏菌攻毒前即刻及攻毒后长达7天的时间点采集粪便样本，并通过16S核糖体RNA（16S rRNA）基因的V1-V3可变区对粪便微生物群进行检测分析。