CIL:1438

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:1437.

兔腰大肌骨骼肌纤维被保持于恒定长度状态，通过浸入甘油诱导其进入肌肉僵直状态。随后将纤维置于Heuser型冷冻按压仪中，通过与液氦冷却的铜块接触实现快速冷冻，再放入Balzers 400型冷冻断裂仪中进行冷冻蚀刻（freeze etching）。以24°角采用铂-碳（Pt-C）对样品进行旋转复型，随后在加速电压为100 keV的JEOL 100CX2型透射电子显微镜（transmission electron microscope）下进行观察。以68000倍的放大倍率将图像记录于柯达（Kodak）4489胶片上，并以±5°倾角拍摄得到立体图像对。使用尼康（Nikon）Coolscan 9000ED扫描仪，以20微米的像素间距对胶片进行扫描。该立体图像对的编号为CIL:1437。