Narrow-band ultraviolet B induces peripheral regulatory T cells to exert antigen-specific immune suppression [scRNA-seq]

Narrow-band ultraviolet B (UVB) is a commonly used therapy for autoimmune diseases. While alloantigen immunization combined with NB-UVB has been shown to induce antigen-specific regulatory T cells (Tregs), the underlying mechanism remains elusive. Single-cell RNA sequencing analysis of patient PBMCs showed that UVB suppressed negative regulators of Treg development, thereby promoting CD4+ T differentiation into Tregs. Overall design: We collected blood samples from psoriasis patients before and after UVB treatmet. Cells were isolated from whole blood to preform single-cell RNA sequencing (scRNA-seq).

窄带紫外线B（NB-UVB）是临床用于治疗自身免疫性疾病的常用手段。尽管已有研究证实，同种异体抗原免疫联合窄带紫外线B（NB-UVB）可诱导抗原特异性调节性T细胞（Tregs），但其潜在作用机制仍未阐明。对患者外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）的单细胞RNA测序分析显示，窄带紫外线B可抑制调节性T细胞发育的负向调控因子，进而促进CD4+ T细胞向调节性T细胞分化。实验设计概况：本研究收集了银屑病患者接受窄带紫外线B治疗前后的血液样本，从全血中分离细胞以开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）。