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Manakins represent a family of small suboscine passerine birds characterized by their elaborate courtship displays, non-monogamous mating system, and sexual dimorphism. This family thus serve as a good model for the study of sexual selection. Here we present the genome assemblies of four manakin species (<em>Cryptopipo holochlora, Dixiphia pipra, Machaeropterus deliciosus</em> and <em>Masius chrysopterus</em>) generated by Single tube-Long Fragment Read (stLFR) technology. The assembly lengths ranged from 1.10 Gb to 1.19 Gb, with average scaffold N50 of 27 Mb and contig N50 of 168 Kb. On average, 9.35% genome region was annotated as repetitive elements, and 12,047 protein-coding genes were annotated in the genomes. We further identified about 75 Mb Z-linked sequences, which contained 583 to 602 genes in each of the four genomes. These manakin genomes will contribute to a deeper understanding of evolutionary history and sexual selection in these species.娇鹟科鸟类（Manakins）是一类小型亚鸣禽雀形目鸟类，以其复杂精致的求偶炫耀（courtship displays）行为、非单配制交配系统与显著的性二态性（sexual dimorphism）为典型特征，因此是开展性选择研究的理想模型类群。本研究报道了4种娇鹟科鸟类的基因组组装结果，其通过单管长片段读取（Single tube-Long Fragment Read，stLFR）技术完成组装，分别为<em>Cryptopipo holochlora</em>、<em>Dixiphia pipra</em>、<em>Machaeropterus deliciosus</em>与<em>Masius chrysopterus</em>。组装后的基因组大小介于1.10 Gb至1.19 Gb之间，平均支架N50（scaffold N50）为27 Mb，平均重叠群N50（contig N50）为168 Kb。平均而言，9.35%的基因组区域被注释为重复序列元件（repetitive elements），每个基因组平均注释得到12047个蛋白质编码基因（protein-coding genes）。本研究进一步鉴定到总长约75 Mb的Z染色体连锁序列（Z-linked sequences），4个基因组中每条此类序列分别包含583至602个基因。本研究获得的娇鹟科鸟类基因组将为深入解析该类群的演化历史与性选择机制提供重要数据支撑。