First dataset - bacterial proteome of carbapanemase-producing Escherichia coli and other Enterobacteriaceae under antibiotic pressure

Antibiotic resistance associated with the expression of the clinically significant carbapenemases, IMP, KPC, and NDM and OXA-48 in Enterobacteriaceae is emerging as a worldwide calamity to health care. In Australia, IMP-producing Enterobacteriaceae is the most prevalent carbapenemase-producing Enterobacteriaceae (CPE). Genomic characteristics of such carbapenemase-producing Enterobacteriaceae (CPE) are well described, but the corresponding proteome is poorly characterised. We have thus developed a method to analyse dynamic changes in the proteome of CPE under antibiotic pressure. Specifically, we have investigated the effect of meropenem at sub-lethal concentrations to develop a better understanding of how antibiotic pressure leads to resistance. Escherichia coli, producing either NDM, IMP or KPC type carbapenemase were included in this study, and their proteomes were analysed in growth conditions with or without meropenem.

与临床重要的碳青霉烯酶（carbapenemases）IMP、KPC、NDM及OXA-48的表达相关的抗生素耐药性，正逐渐演变为全球医疗保健领域的重大公共健康危机。在澳大利亚，产IMP型碳青霉烯酶的肠杆菌科（Enterobacteriaceae）是最为流行的产碳青霉烯酶肠杆菌科（carbapenemase-producing Enterobacteriaceae, CPE）。目前，此类产碳青霉烯酶肠杆菌科的基因组特征已得到充分阐释，但其对应的蛋白质组学特征却鲜有系统表征。为此，本研究开发了一种可用于分析抗生素压力下产碳青霉烯酶肠杆菌科蛋白质组动态变化的方法。具体而言，本研究探究了亚致死浓度美罗培南（meropenem）的作用效果，以期更深入地理解抗生素压力如何诱导耐药性的产生。本研究纳入了携带NDM、IMP或KPC型碳青霉烯酶的大肠杆菌（Escherichia coli），并在添加与不添加美罗培南的两种培养条件下对其蛋白质组进行了分析。