Differentiation of Inflammation-responsive Astrocytes from Glial Progenitors Generated from Human Induced Pluripotent Stem Cells. Homo sapiens

The goals of this study are to generate inflammation-sensitive astrocytes from human induced pluripotent stem cells. We examine the transcriptomic inflammatory signature of generated astrocytes following Il1Beta exposure. Primary human cerebellar astrocytes, human induced pluripotent stem cells (hiPSC)-derived neural precursor cells (NPCs) and hiPSC-derived astrocytes were treated with Il1beta and compared to vehicle treated controls. Results: hiPSC-derived can be differentiated to astrocytes that are inflammation sensitive. Overall design: Primary human cerebellar astrocytes, human induced pluripotent stem cells (hiPSC)-derived neural precursor cells (NPCs) and hiPSC-derived astrocytes were treated with Il1beta and compared to vehicle treated controls.

本研究的核心目标为通过人类诱导多能干细胞（human induced pluripotent stem cells, hiPSC）制备炎症敏感性星形胶质细胞，并检测经白细胞介素1β（Il1Beta）刺激后所获星形胶质细胞的转录组炎症特征谱。
实验处理方案为：将原代人小脑星形胶质细胞、人类诱导多能干细胞（hiPSC）来源的神经前体细胞（neural precursor cells, NPCs）以及hiPSC来源的星形胶质细胞用Il1beta进行刺激，并以溶剂处理组作为对照开展比对分析。
结果：人类诱导多能干细胞可分化为炎症敏感性星形胶质细胞。
实验整体设计：将原代人小脑星形胶质细胞、人类诱导多能干细胞（hiPSC）来源的神经前体细胞（NPCs）及hiPSC来源的星形胶质细胞用Il1beta处理，并与溶剂处理的对照组进行比对。