Table_2_Comprehensive Analysis of Long Non-coding RNA Modulates Axillary Bud Development in Tobacco (Nicotiana tabacum L.).XLSX

Long non-coding RNAs (lncRNAs) regulate gene expression and are crucial for plant growth and development. However, the mechanisms underlying the effects of activated lncRNAs on axillary bud development remain largely unknown. By lncRNA transcriptomes of axillary buds in topped and untopped tobacco plants, we identified a total of 13,694 lncRNAs. LncRNA analysis indicated that the promoted growth of axillary bud by topping might be partially ascribed to the genes related to hormone signal transduction and glycometabolism, trans-regulated by differentially expressed lncRNAs, such as MSTRG.52498.1, MSTRG.60026.1, MSTRG.17770.1, and MSTRG.32431.1. Metabolite profiling indicated that auxin, abscisic acid and gibberellin were decreased in axillary buds of topped tobacco lines, while cytokinin was increased, consistent with the expression levels of related lncRNAs. MSTRG.52498.1, MSTRG.60026.1, MSTRG.17770.1, and MSTRG.32431.1 were shown to be influenced by hormones and sucrose treatments, and were associated with changes of axillary bud growth in the overexpression of NtCCD8 plants (with reduced axillary buds) and RNA interference of NtTB1 plants (with increased axillary buds). Moreover, MSTRG.28151.1 was identified as the antisense lncRNA of NtTB1. Silencing of MSTRG.28151.1 in tobacco significantly attenuated the expression of NtTB1 and resulted in larger axillary buds, suggesting the vital function of MSTRG.28151.1 axillary bud developmen by NtTB1. Our findings shed light on lncRNA-mRNA interactions and their functional roles in axillary bud growth, which would improve our understanding of lncRNAs as important regulators of axillary bud development and plant architecture.长链非编码RNA（long non-coding RNAs，lncRNAs）可调控基因表达，对植物生长发育至关重要。然而，激活的长链非编码RNA对腋芽发育的调控机制目前仍未完全阐明。本研究通过对打顶与未打顶烟草植株腋芽的长链非编码RNA转录组进行分析，共鉴定得到13694条长链非编码RNA。长链非编码RNA分析显示，打顶促进腋芽生长的效应，可能部分由差异表达的长链非编码RNA反式调控的激素信号转导与糖代谢相关基因所介导，涉及的差异长链非编码RNA包括MSTRG.52498.1、MSTRG.60026.1、MSTRG.17770.1及MSTRG.32431.1。代谢组分析结果显示，打顶烟草植株腋芽中的生长素、脱落酸与赤霉素含量均下调，而细胞分裂素含量上调，该变化与相关长链非编码RNA的表达水平一致。实验证实，MSTRG.52498.1、MSTRG.60026.1、MSTRG.17770.1及MSTRG.32431.1的表达可受激素与蔗糖处理调控，且在NtCCD8过表达（腋芽数量减少）与经RNA干扰（RNA interference）靶向NtTB1的植株（腋芽数量增加）中，这些长链非编码RNA的表达变化与腋芽生长状态的改变显著相关。此外，本研究鉴定得到MSTRG.28151.1为NtTB1的反义长链非编码RNA。对烟草中MSTRG.28151.1进行沉默，可显著抑制NtTB1的表达并导致腋芽体积增大，提示MSTRG.28151.1可通过调控NtTB1的表达参与腋芽发育过程。本研究结果揭示了长链非编码RNA与信使RNA的相互作用及其在腋芽生长中的功能角色，有助于深化我们对长链非编码RNA作为腋芽发育与植物株型重要调控因子的认知。