Molecular and technological characterization of lactic acid bacteria isolated from artisanal fresh cheeses: Raw data, images, and statistical analyses

This dataset supports the article “Molecular and technological characterization of lactic acid bacteria isolated from artisanal fresh cheeses.”It includes a compressed folder (LAB_dataset_REPOSITORY.zip) containing original and processed data from the molecular, technological, and inhibitory characterization of lactic acid bacteria (LAB) isolated from artisanal cheeses. The dataset comprises electrophoresis gel images (ARDRA, RAPD, DNA extraction, and 16S rRNA sequencing), raw images from technological assays (fermentation, proteolytic activity, β-galactosidase activity, lipolysis, diacetyl production, milk acidification, and salt tolerance), as well as Excel spreadsheets with quantitative data, sequence files (.FASTA, .BIO), and Python scripts for statistical analysis.All materials were organized to ensure transparency, reproducibility, and reusability in studies related to dairy biotechnology, microbial diversity, and the development of starter and bioprotective cultures. Funding and acknowledgments: This work was supported by Fondo de CTeI del Sistema General de Regalías de Colombia through the project BPIN 2017000100114 and the University of Nariño. The authors acknowledge the entities of Agreement DPJ-209364: Center for Research and Technological Development in Applied Sciences (CIDTCA), Departmental Health Institute of Nariño (IDSN), and the Local Development Agency of Nariño (ADEL).本数据集配套发表于论文《手工鲜酪中分离乳酸菌的分子与技术表征》。数据集包含压缩包LAB_dataset_REPOSITORY.zip，其中收录了从手工鲜酪中分离的乳酸菌（lactic acid bacteria, LAB）的分子表征、技术表征及抑菌活性表征相关的原始与处理后数据。数据集涵盖电泳凝胶图像（包括ARDRA、RAPD、DNA提取及16S rRNA测序相关凝胶图）、技术检测实验的原始图像（涵盖发酵、蛋白水解活性、β-半乳糖苷酶活性、脂肪分解、双乙酰生成、牛乳酸化及耐盐性检测），同时包含携带定量数据的Excel电子表格、序列文件（.FASTA、.BIO）以及用于统计分析的Python脚本。所有材料均已完成系统化整理，以保障乳品生物技术、微生物多样性研究以及发酵剂与生物保护培养物开发相关研究的透明度、可重复性与可复用性。 资助与致谢：本研究受哥伦比亚一般特许权体系CTeI基金通过项目BPIN 2017000100114及纳里尼奥大学资助。作者感谢协议DPJ-209364涉及的相关机构：应用科学研究与技术发展中心（CIDTCA）、纳里尼奥省卫生研究所（IDSN）以及纳里尼奥地方发展局（ADEL）。