Neuronal subtype expression profiles from adult mouse cortex

Neurons were dissociated and purified from adult (~3 months) mouse brains. We used four strains with genetic markers that enabled live sorting. Thy1-GFP labels a subset of excitatory neurons; VIP-tdTomato, SST-tdTomato and PVALB-tdTomato labels different subsets of inhibitory neurons. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0016304 RNA from different neuronal subtypes sorted from adult mouse cortex using fluorescence activated cell sorting本研究从成年（约3月龄）小鼠脑组织中解离并纯化神经元，采用四种携带可用于活细胞分选的遗传标记的小鼠品系。其中Thy1-GFP可标记一类兴奋性神经元亚群；VIP-tdTomato、SST-tdTomato与PVALB-tdTomato可分别标记不同亚型的抑制性神经元亚群。"SAMPLE_ID"为该数据集的样本特征字段，为基因泰克（Genentech）内部使用的样本标识符。本项目在基因泰克的ExpressionPlot数据库中的编号为PRJ0016304；本数据集的RNA样本取自通过荧光激活细胞分选（fluorescence activated cell sorting）从成年小鼠大脑皮层分离得到的不同神经元亚型。