The role of DELLA protein in tomato plant response to water-deficit stress

We previously showed that increased levels of the tomato DELLA protein PROCERA (PRO) promoted ABA responses in guard cells, including ABA-induced stomatal closure and gene expression. Thus we aimed to identifiy DELLA-regulated genes in guard cells in order to study the molecular mechanism by which DELLA promotes stomatal closure. Overall design: Transcriptome analysis of guard cells isolated from plants expressing DELLA gain-of-function (pro?17) or from DELLA loss-of-function mutant (pro)

我们此前的研究表明，番茄DELLA蛋白PROCERA（PRO）表达水平升高可增强保卫细胞对脱落酸（ABA）的响应，包括ABA诱导的气孔关闭与基因表达。因此本研究旨在鉴定保卫细胞中受DELLA调控的基因，以解析DELLA促进气孔关闭的分子机制。实验整体设计：对从表达DELLA功能获得型突变体（pro?17）以及DELLA功能缺失型突变体（pro）的植株中分离得到的保卫细胞开展转录组分析。