Expression data from memory P14 transgenic T cells. Mus musculus

Microarray analysis was used to compare differences and similarities in gene expression patterns between memory CD8+ T cells from mice infected with lymphocytic choriomeningitis virus (LCMV) or influenza virus. Keywords: infection response Overall design: Mice containing transferred P14 CD8+ transgenic T cells were infected with LCMV or influenza and rested for 90 days. P14 cells were purified by cell sorting, as well as cells from unimmunized P14 mice as controls, and used for RNA extraction and hybridization on Affymetrix microarrays.本研究采用基因微阵列分析技术，对比感染淋巴细胞脉络丛脑膜炎病毒（Lymphocytic choriomeningitis virus, LCMV）或流感病毒的小鼠来源的记忆性CD8+ T细胞的基因表达模式差异与相似性。 关键词：感染应答 实验设计概述：将P14 CD8+转基因T细胞移植至小鼠体内，随后分别感染LCMV或流感病毒并静置饲养90天。通过细胞分选纯化小鼠体内的P14细胞，同时以未免疫的P14小鼠的细胞作为对照，随后提取RNA并在Affymetrix基因微阵列芯片上完成杂交实验。