Identification of differentially expressed miRNAs in drug resistant and drug sensitive breast cancer tissues. Homo sapiens

To identify miRNAs involved in drug resistance of human breast cancer, a miRNA microarray was performed on 5 cases of drug resistant tissues and 5 cases of drug sensitive tissues.The expression levels of totally 2019 miRNAs in 5 pairs of matched, drug resistant and drug sensitive tissues were examined by microarray. There were 27 differentially expressed miRNAs between drug resistant and drug sensitive tissues were identified of which there were 11 significantly up-regulated while the other 16 were down-regulated in drug resistant tissues compared to drug sensitive tissues. It was found that miR-489 was one of the most downregulated miRNAs in drug resistant tissues. Overall design: Group 1: Examination of miRNAs in drug resistant tissues (5 cases), including C100, C17, C28, C22 and C115. Group 2: Examination of miRNAs in drug sensitive tissues (5 cases), including H801, H31, H19, H20, and H32.

为鉴定参与人类乳腺癌耐药过程的微小RNA（microRNA，miRNA），研究团队对5例耐药乳腺组织与5例敏感乳腺组织开展了miRNA芯片检测。
本研究通过芯片检测了5对匹配的耐药、敏感组织样本中总计2019个miRNA的表达水平。
研究共鉴定出27个在耐药组织与敏感组织间存在差异表达的miRNA，其中相较于敏感组织，耐药组织中有11个miRNA显著上调，剩余16个显著下调。
研究发现，miR-489是耐药组织中下调幅度最显著的miRNA之一。
总体实验设计：组别1：耐药组织miRNA表达谱检测（共5例，样本编号为C100、C17、C28、C22及C115）；组别2：敏感组织miRNA表达谱检测（共5例，样本编号为H801、H31、H19、H20及H32）。