Targeted RNA Sequencing of a panel of ovarian cancer cell lines

Assess the baseline expression of the genes in the Oncology Biomarker Panel across the panel of ovarian and cervical cancer cell lines. This data will then be compared to the sensitivity of the cell lines to DDR inhibitors. The expression data will be correlated with the sensitivity of the cell lines to see whether determinants of sensitivity described in the literature translate. A hierarchy of genes will be determined. It is expected that the cells that are more sensitive to DDR inhibitors will have decreased expression of the genes known to be determinants of sensitivity.本研究将对卵巢癌与宫颈癌癌细胞系面板中，肿瘤生物标志物组（Oncology Biomarker Panel）内各基因的基础表达水平进行检测。随后将上述表达数据与各癌细胞系对DDR抑制剂（DDR Inhibitors）的敏感性开展对比分析，并通过关联表达数据与癌细胞系敏感性，验证文献中报道的敏感性决定因素是否可在本研究中得到印证。同时将明确各基因的表达层级关系，预期对DDR抑制剂敏感性更高的癌细胞系，其作为敏感性决定因素的已知基因的表达水平将呈现下调趋势。