RNA sequencing of wild type and Thpo deficient hematopoietic stem cells

RNA sequencing was conducted on up to 5000 CD34-CD150+CD48-LSK cells from Thpo+/+ , Thpo-/- along with Thpo+/+ and Thpo-/- mice treated with 10 days of Thpo receptor agonist (Romiplostim) Overall design: HiSeq4000 sequencing (Illumina) platform (150bp paired-end). Three samples from a single group were placed on single run to produce more than 8x107 reads per sample

本研究针对来源于Thpo+/+、Thpo-/-小鼠，以及经10天促血小板生成素（Thrombopoietin, Thpo）受体激动剂罗米司亭（Romiplostim）处理的Thpo+/+与Thpo-/-小鼠的至多5000个CD34⁻CD150⁺CD48⁻LSK细胞，开展了RNA测序（RNA sequencing）。
实验设计：采用Illumina HiSeq4000测序平台进行150bp双端测序；每组设置3个生物学样本并于同一次测序流程上机，单样本测序产出reads数均超过8×10⁷条。