Desert soil culture-enriched metagenomics

This project aims to investigate the taxonomic and functional responses of desert soil microbiomes to thermal stress by integrating large-scale culture-enriched metagenomics with genome-resolved analysis. We focused on two representative desert soil types from the Gurbantunggut Desert, including rhizosphere and bulk soils, and subjected them to 90 distinct culture conditions varying in media types and incubation temperatures. A total of 92 metagenomic datasets, including two from original soils and 90 from culture-enriched samples, were generated and analyzed. Using a standardized pipeline, we reconstructed 1,258 metagenome-assembled genomes (MAGs), including a large number of high-quality cultivated MAGs (cMAGs), and explored their taxonomic composition, co-occurrence patterns, and metabolic profiles. This study provides novel insights into the ecological adaptation and functional reorganization of desert bacterial communities under temperature stress, and delivers a valuable genomic resource for future microbial ecology and climate-related research.本研究旨在通过整合大规模富集培养宏基因组学与基因组解析分析，探究荒漠土壤微生物组对热胁迫的分类学与功能响应。我们选取古尔班通古特沙漠（Gurbantunggut Desert）的两种典型荒漠土壤类型——根际土壤与非根际土壤（bulk soil），并设置了90种不同培养基类型与培养温度组合的培养条件。共生成并分析了92套宏基因组数据集，其中2套源自原始土壤样品，90套源自富集培养样品。通过标准化分析流程，我们重构了1258个宏基因组组装基因组（metagenome-assembled genomes，MAGs），其中包含大量高质量可培养宏基因组组装基因组（cultivated MAGs，cMAGs），并解析了它们的分类组成、共现模式与代谢谱。本研究为温度胁迫下荒漠细菌群落的生态适应与功能重塑提供了全新见解，同时为后续微生物生态学与气候相关研究提供了宝贵的基因组资源。