RNAseq of wild Brassica oleracea species following Sclerotinia-infection

Oilseed rape (Brassica napus) is one of the most important oil-producing crops worldwide. The narrow gene pool of oilseed rape hampers its resistance breeding. Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is one of the most destructive diseases in many oilseed rape growing regions, worldwide. So far, no effective genetic source of resistance to S. sclerotiorum in B. napus germplasm is available, and yet knowledge of molecular plant-fungal interactions is limited. We performed RNAseq of a resistant (B. villosa; B1896) and a susceptible (B. oleracea; B1909) wild Brassica oleracea species at a early stage of Sclerotinia-infection for a comparative transcriptome profiling. The data provides novel insights into the early defense response of a unique genetic source for quantitative resistance against Sclerotinia.

甘蓝型油菜（Brassica napus）是全球最重要的油料作物之一。其狭窄的基因库严重制约了该作物的抗病育种工作。由核盘菌（Sclerotinia sclerotiorum）引发的菌核茎腐病（Sclerotinia stem rot, SSR）是全球众多油菜种植区中破坏性最强的病害之一。截至目前，在甘蓝型油菜的种质资源中尚未发现针对核盘菌的有效抗病遗传资源，且学界对植物与真菌互作的分子机制认知仍较为匮乏。本研究在菌核菌侵染早期，对2份野生芸薹属甘蓝材料——抗病材料（B. villosa；B1896）与感病材料（B. oleracea；B1909）开展了RNA测序（RNA-seq），以进行比较转录组分析。该数据集为解析针对核盘菌的独特数量抗性遗传资源的早期防御应答机制提供了全新见解。